Field inversion gel electrophoresis in denaturing polyacrylamide gels

Silver staining of denaturing gradient gel electrophoresis gels.

and -IgM k and l light chains did not show the presence of monoclonal components. Reduction with mercaptoethanol (1:2 dilution), which dissociates complexes but does not dissociate albumin dimers, excluded the possible formation of complexes with a1-antitrypsin. Interferences by radio-opaque agents or medications, which could lead to the appearance in the CZE electropherograms of abnormal peaks...

Polyacrylamide Gel Electrophoresis

Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...

Neutral polyacrylamide gel electrophoresis.

MATERIALS 5x TBE electrophoresis buffer Polyacrylamide gels are poured and run in 0.5x or 1x TBE at low voltage (1-8 V/cm) to prevent denaturation of small fragments of DNA by Joulic heating. Other electrophoresis buffers such as 1x TAE (please see Agarose Gel Electrophoresis) can be used, but they are not as good as TBE. The gel must be run more slowly in 1x TAE, which does not provide as much...

Using a Temperature Gradient against the Time in Polyacrylamide Gel Electrophoresis May Eliminate the Need for Stacking Gels

Background and Objectives: Making stacking gels for polyacrylamide gels in the laboratory by conventional methods is laborious and time consuming. Considering the role of temperature in polyacrylamide gels with respect to electrical resistance and viscosity, we assumed that decreasing the temperature would cause an increase in electrical resistance and viscosity.  Ultimately, a downward tempera...

Lipoprotein X: BIOCHEMICAL PREDICTORS AND DETECTION BY NON-DENATURING POLYACRYLAMIDE GRADIENT GEL ELECTROPHORESIS